Tutorials: http://www.nmr.chem.uu.nl/~abonvin/tutorials/Assignment-Data/assignment.html http://www.nmr2.buffalo.edu/nesg.wiki/Resonance_Assignment/Sparky

Sparky NMR Analysis Quick Guide

Finding and Loading Spectra:

• Navigate to your NMR data directory and execute find . -name 2rr to locate all 2D spectrum files (TOCSY, ROESY, NOESY).
• Load the identified files into Sparky for analysis.

Viewing Options and Pointer Modes:

• Access spectrum viewing options by vt shortcut or through View -> View options. Alternatively, right-click inside a spectrum window.
• Default pointer mode can be initiated via View -> Pointer Modes or with F1-F12 keys.

Peak Selection and Manipulation:

• Select peaks using F8.
• Adjust contour levels with the ct shortcut to modify signal visibility.
• Move or delete peaks by switching to “select” mode, using the mouse to drag or select, and pressing the delete key to remove peaks.
• Undo peak deletions with eu.

Peak Labeling and Copying:

• To label a peak, type at and fill in the details in the prompt for residue and atom assignment.
• Copy peaks with assignments using oc (ornament copy), then paste using op in another spectrum window.

Spectrum Overlay and Printing:

• Overlay spectra using ol after ensuring axes are named identically.
• Print contour plots to a PostScript printer with pt; save outputs directly if supported.

Peak Assignment Commands:

• Assign or select peaks using specific commands like pa for all peaks, pF for fully assigned, or pL for long range assignments.
• Change peak color selection with pC and invert selections with pI.

Miscellaneous Commands:

• Display axis atom names with xa.
• Duplicate spectrum views for comparison with vd.
• Adjust label font sizes in contour plots using oz.
• For precise plotting dimensions, refer to Plotting spectra with Sparky.

View Management:

• Hide or reveal views using vh to hide, or select by name from the View menu to reveal.
• Manage crowded screen space by iconifying or maximizing views as needed.

Additional Resources:

• For chemical shift statistics and starting points for assignments, check commands under shift.tcl.