NMR Experiments Recommendation Questionnaire

1. What is the highest concentration you can achieve for your protein sample? (mg/mL)

2. What buffer type and pH are you using for your protein sample?

3. At the given concentration, is your protein stable in terms of aggregation, degradation and conformational changes? Yes No

4. Does your protein have any of the following features? (Answer each feature separately as yes or no.)

4.1 Intrinsically Disordered Regions: Yes No

4.2 Long Loops (>10 amino acids): Yes No

4.3 Flexible Termini (>6 amino acids): Yes No

5. Is your protein embedded in micelles? Yes No

6. Are there any ligands, co-factors, or nucleotides present in your protein sample? Yes No

7. What are your desired outcomes from the analysis? (Select one)

7.1 Only backbone chemical shift assignments

7.2 Backbone and side chain atom chemical shift assignments (currently only aliphatic atoms)

7.3 Ensemble of 3D protein structures